Journal article
Spectroscopic Characterization and Differentiation of SARS-CoV-2 Virus-like Particles
A Dodla, M Giergiel, A Mclean, K Kochan, L Earnest, MA Edeling, JL McAuley, DI Godfrey, DFJ Purcell, AHY Yap, JC Montoya, JA Roberts, S Collett, S Shukla, S Saxena, J Torresi, BR Wood
Analytical Chemistry | American Chemical Society (ACS) | Published : 2025
Abstract
Virus-like particles (VLPs) are recombinant, noninfectious, self-assembled structures that are made up of the viral structural proteins that mimic the morphology of viruses but lack genomic material. VLPs have been used to develop vaccines against viruses and cancer, leading to a surge of industry interest in exploring VLP vaccines. There are strict quality controls as a part of downstream processing in the production of nonreplicating VLPs. We characterized SARS-CoV-2 VLPs of the Beta and Omicron BA.5 subvariants, which differ in 43 amino acids in the spike protein. By comparing the Raman spectra of these particles with those of SARS-CoV-2 virions and purified RNA isolated from yeast, we co..
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Grants
Awarded by Department of Biotechnology, Ministry of Science and Technology, India